![]() Not a scientist? NO access to a lab? NO PROBLEM! We will use materials you can find around the house, at the dollar store and of course Amazon. SHORT VERSION OF THE EXPERIMENT (tl dr): you will check for “germs” in different places around a school to see which surfaces grow the most bacteria. Conclusions: what we learned from our science fair project? Did it work?.Data Analysis – what does the data tell us?.Methods/Procedure – How we’re going to complete our science fair project:.Great detail…but where do I get plates? Two options: make them or purchase them.Designing the Experiment: picking a hypothesis.Who can do this science fair project? Age range: 3rd Grade and up.Streaking bacterial plates – how we grow bacteria.Science fair project overview: what we’ll do.In general I would give things 15 minutes and 15 psi (121 centigrade) to be sure. If you want to go further with 'home microbiology' I suggest you get hold of a pressure cooker and learn how to use it to sterilise things. These produce lots of spores that are quite allergenic and some can even cause respiratory infections so take care not to inhale these. Looking at your plates there are a lot of sporulating fungi evident. This proves that the colonies developed from microbes in the inoculum rather than contaminants in the medium. This would allow you to assess the quality of your sterilization and aseptic technique, The hypothesis that both sterilization and aseptic technique were both perfect would suggest that no microbial colonies should be found on the control plates while colonies develop on the inoculated plates. ![]() Nice work! I would have included a Petri dish of medium that was not inoculated at the start but incubated along side your experimental plates. ![]() Take your sample over the sink and start pouring bleach over it making sure to not let any of the bleach touch your skin, this should kill the bacteria left over, after that place it in a sealed plastic bag and then dispose of it in the trash. Store your sample in a warm and dark place where the bacteria can develop well for the next 5-7 days, and don't forget to store the dishes upside down, so the bacterial growth remains undisturbed by any water droplets that may form on the top of the Petri dish.Īfter 5-7 days have passed you should be able to see many types of bacteria, mold, and fungi have grown in the agar, if you would like, you can examine the cultivated sample using a pair of tweezers with the utmost caution to not touch or inhale the bacteria as it can pose a risk to you.Īfter conducting your experiments on the sample, it is time to dispose of it as it is no longer of use to you, the correct way to dispose of the sample is by wearing rubber gloves, an apron, a mask, and goggles to prevent any bacteria touching you. Immediately close the lid on the Petri dish to prevent contamination of the sample. After collecting your sample take the sterile swap and rub it directly on the hardened agar to introduce the sample to the agar. then quickly cover the dish with the top part to prevent airborne bacteria from contaminating the sample, and set the agar to cool for 30 minutes - 2 hours.Īfter the agar hardens, take out your sterile swabs and collect a sample of bacteria by rubbing the swab on the surface of your choice. Take out your Petri dish and sterilize it all over, then pour your agar solution into the bottom half of your Petri dish, pour just enough to cover the bottom and a little more. Next, place the bowl in the microwave, and let it boil for a minute, make sure it doesn't boil over (you should know it's done when the solution turns clear). The first step is to take your bowl and mix in it 1.2 grams of agar powder and 60 milliliters of hot water until combined. ![]() Now that you have all the materials ready its time to start the experiment. ![]()
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